Skip to main content
. 2017 Sep 8;12(9):e0184308. doi: 10.1371/journal.pone.0184308

Fig 5. EXL-1::GFP nuclear translocation in intestine occurs in an ordered manner.

Fig 5

(A1-A3): At 20°C, EXL-1::GFP has basal nuclear accumulation at the most posterior region (arrow in A1). (B1-B4): After heat shock at 35°C for 1 hour, EXL-1::GFP nuclear accumulation still remains in the most posterior region. B4 is enlarged image of the rectangular part of B1. (C1-C4): After 2 hour of heat shock, EXL-1::GFP nuclear translocation mostly occurs between middle region (around vulva) and tail region. C4 is enlarged image of the rectangular part of C1. (D1-D3): After 3 hours of heat shock, EXL-1::GFP nuclear translocation appears in the middle region and posterior region. (E1-E3): Enlarged images show EXL-1::GFP nuclear translocation in the middle region of intestine after 3 hours of heat treatment. (F1-F3): After 4 hours of heat shock, EXL-1::GFP nuclear translocation was observed in the whole intestinal region from anterior to posterior. (G1-G3): Enlarged images of EXL-1::GFP nuclear translocation at anterior region of intestine after 4 hours of heat treatment. Image A1-3, B4, C4, E1-3, G1-3 are taken with 400 magnification power; all the other images are taken with 100 magnification power; (H): Quantification of nuclear to cytoplasmic fluorescence ratio under different heat shock treatments.