Table 4. JPred4 predicted solvent accessibility for S/T in the MSS and USS datasets.

The proportions of buried S/T as predicted by the Jnetsol method in JPred4. The proportions of buried S/T are significantly smaller for modified group.

	Modified (MSS)		Unmodified (USS)		p value
Buried at	Proportion (n)	95% CI [lower, upper]	Proportion (n)	95% CI [lower, upper]
0%	0.01 (7)	[0.00, 0.01]	0.01 (836)	[0.008, 0.009]	0.18
5%	0.04 (55)	[0.03, 0.05]	0.04 (3,917)	[0.038, 0.040]	0.86
25%	0.29 (403)	[0.27, 0.31]	0.35 (28,044)	[0.27, 0.28]	0.31

95% CI– 95% confidence interval; n–number of S/T predicted to be buried. The p value refers to the two-tailed z-score test between the modified and unmodified groups.