Table 6. Predicted disorder between modified and unmodified S/T.

All disorder prediction methods, excepting DisEMBL-HOTLOOPS, reveal a small but significant increase of mean disorder score for modified S/T over unmodified ones.

Method	Mean score modified (MSS) ± SE	Mean score unmodified (USS)± SE	p value
DisEMBL-REM465	0.48 ± 0.004	0.47 ± 0.001	0.01
DisEMBL-COILS	0.60 ± 0.004	0.58 ± 0.001	<0.01
DisEMBL-HOTLOOPS	0.10 ± 0.001	0.10 ± 0.001	0.45
IUpred-Long	0.59 ± 0.006	0.55 ± 0.001	<0.01
IUpred-Short	0.48 ± 0.005	0.45 ± 0.001	<0.01
JRonn	0.62 ± 0.004	0.61 ± 0.001	0.02

The p value refers to the two-tailed t-test between the modified and unmodified groups. SE–standard error.