Table 5. Fluorescence lifetimes (τ) and relative representations for two different fluorescence probes and hyaluronan of different molecular weights (values in italics correspond to scattered light).
blank | freeze-dried | |||
---|---|---|---|---|
τ (ns) | representation | τ (ns) | representation | |
prodan | ||||
Mw = 106 kDa | 0.64 | 51% | 0.01 | 77% |
1.92 | 49% | 0.95 | 12% | |
2.79 | 11% | |||
Mw = 420 kDa | 0.60 | 55% | 0.02 | 74% |
2.00 | 45% | 1.04 | 17% | |
3.54 | 9% | |||
Mw = 1.46 MDa | 0.62 | 50% | 0.02 | 75% |
1.88 | 50% | 0.87 | 14% | |
2.61 | 11% | |||
perylene | ||||
Mw = 106 kDa | 0.05 | 68% | ||
no data | 3.91 | 24% | ||
7.29 | 8% | |||
Mw = 420 kDa | 0.05 | 62% | ||
no data | 3.44 | 27% | ||
6.52 | 11% | |||
Mw = 1.46 MDa | 0.05 | 68% | ||
no data | 3.55 | 20% | ||
6.99 | 12% |