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. 2017 Sep 8;12(9):e0184732. doi: 10.1371/journal.pone.0184732

Fig 1. Regulation of TNF expression after TCR stimulation.

Fig 1

A) Expression of IFNγ and TNF was tracked at 0, 5, 24, 48 and 72 h after TCR engagement with anti-CD3 mAb with co-activation of co-stimulatory signals using intracellular cytokine staining with the frequency of CD8+ T cells expressing each cytokine indicated. B) Deposition of the permissive mark, H3K4me3, or the repressive mark, H3K27me3, or C) RNA polymerase II enrichment at Ifng or Tnf PPR at 0, 5 or 48 h after stimulation with anti-CD3/CD8/CD11a/CD28. Line graphs represent mean+/-SEM and data are representative of two independent experiments with n = 4.