Fig 3. Impact of intrinsic and extrinsic TNF on CD8⁺ T cell responses.

OT-I WT or OT-I Tnf-/- T cells were transferred into either WT or Tnf-/- recipients followed by infection with HKx31-OVA. A) Schematic of transfer of 1 x 10⁴ donor OT-I WT or Tnf-/- CD8 T cells into WT or Tnf-/- mice, alongside WT and Tnf-/- control mice that did not receive transferred OT-I cells (No Tx). B) Proportion and C) absolute number of CD8⁺ T cells that are OVA-specific using tetramer staining in spleen of recipient mice at d 10 after primary IAV infection. D) Proportion and CD8⁺ T cells that are OVA-specific using tetramer staining in the BAL. Bar graphs represent mean+/-SEM, data are representative of 3 independent experiments (n = 4–5), * p≤0.05, ** p≤0.01, NS indicates not significant, using Mann-Whitney test with Bonferroni correction for multiple comparisons.