Fig 1. Selected image slices from a stack of 81 slices acquired from MCF-7 cells treated by doxorubicin at a dose of 10μM for 48 hours.

Each panel consists of red (upper left), green (upper right), blue (lower left) channels and merged (lower right) with slice number marked. The brightness of blue channels are increased in slices 30 and 35 to make cell membranes stained by Annexin V more visible. This stack contains only 1 viable cell (white arrow) defined by the total blue channel fluorescence less than background value. Bar = 20μm.