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. 2017 Sep 8;12(9):e0184582. doi: 10.1371/journal.pone.0184582

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© 2017 Tan, Nusse

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — (A) The average number of cells per cluster is plotted for K5-only (red), DP-only (yellow) and mixed (green) cluster types at each time point. The differences in average cluster sizes between 48 hour trace and 6 month or 1 year trace are tested for significance. (B and C) The size distribution of K5-only clusters (B) and DP-only clusters (C) at each time point. (D and E) Representative images of mGFP-labeled K5-only clusters (D) and DP-only clusters (E) observed at 48 hours, 1 month, 3 months and 1 year after trace initiation. The left panels show co-staining with mGFP, K5 and the K8, and the right panels show the same clusters with DAPI. Graphs depict averages ± SEM, and two-tailed unpaired Student’s t-test was performed to obtain p values of significance. ns—not significant, ** p<0.01. Scale bars represent 20μm.