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. 2017 Sep 8;12(9):e0184505. doi: 10.1371/journal.pone.0184505

Table 3. Mascot results of identified and non-identified proteins found in 2-DE electrophoresis gels of CON cultures.

Summary of the Mascot search results of identified (shaded grey) (high score and/or high sequence coverage) and non-identified proteins (low score) found in 2-DE electrophoresis gels of CON cultures after 4 and 15 days of incubation and analyzed by MALDI TOF/TOF MS/MS. The “x” indicates proteins present in each condition. The lower the expectation value (e-value), the more significant is the score.

Spot ID Protein description Day Protein accession Microorganism Mass (Da) Protein Score Score > e-value Order AM
4 15
2 peroxidase x gi|913648761 Achromobacter piechaudii 23911 108 87 0.0004 Burkholderiales
4 flagellar motor protein MotB x gi|503612114 Sphingobium chlorophenolicum 37434 102 87 0.0017 Sphingomonadales x
5 hemerythrin x x gi|739609734 Sphingobium quisquiliarum 19421 234 90 2E-16 Sphingomonadales x
13 MULTISPECIES: molecular chaperone GroEL x gi|493430365 Achromobacter 57416 190 87 2.7E-12 Burkholderiales
14 molecular chaperone GroEL x x gi|493268672 Sphingobium chlorophenolicum 57331 194 90 0.00017 Sphingomonadales x
15 MULTISPECIES: glutathione S-transferase x x gi|158346886 Sphingomonadaceae family 21470 234 90 2E-16 Sphingomonadales x
19 MULTISPECIES: anthranilate 1,2-dioxygenase small subunit x x gi|496103626 Sphingomonadaceae family 18116 185 86 7.7E-12 Sphingomonadales x
30 MULTISPECIES: glutathione S-transferase x x gi|158346886 Sphingomonadaceae family 21470 191 87 2.1E-12 Sphingomonadales x
100 OmpA/MotB domain protein x gi|307294285 Sphingobium chlorophenolicum L-1 37434 96 81 0.002 Sphingomonadales
102 acid-coenzyme A ligase x gi|317402668 Achromobacter xylosoxidans C54 60191 93 81 0.004 Burkholderiales
103 chaperone protein DnaK x gi|311104375 Achromobacter xylosoxidans A8 69572 103 81 0.00038 Burkholderiales
105 seryl-tRNA synthetase x gi|307294312 Sphingobium chlorophenolicum L-1 46894 105 81 0.00024 Sphingomonadales x
106 translation elongation factor Tu 1 x gi|311109614 Achromobacter xylosoxidans A8 42919 105 81 0.00024 Burkholderiales
107 10 kDa chaperonin, GroES x gi|94497511 Sphingomonas sp. SKA58 10252 91 81 0.0055 Sphingomonadales x
108 benzene dioxygenase small subunit x gi|158346882 Sphingomonas sp. LH128 18996 191 81 6E-13 Sphingomonadales x
110 aconitate hydratase 1 x x gi|307293228 Sphingobium chlorophenolicum L-1 96376 104 81 0.0003 Sphingomonadales x
111 chaperonin x gi|317405440 Achromobacter xylosoxidans C54 57416 138 81 1.2E-07 Burkholderiales
113 putative oxidoreductase x gi|53720358 Burkholderia pseudomallei K96243 23904 90 81 0.0081 Burkholderiales
201 aconitate hydratase 1 x x gi|307293228 Sphingobium chlorophenolicum L-1 96376 167 81 1.5E-10 Sphingomonadales x
202 10 kDa chaperonin, GroES x gi|94497511 Sphingomonas sp. SKA58 10252 94 81 0.003 Sphingomonadales x
203 hypothetical protein SJA_C1-23830 x gi|294012369 Sphingobium japonicum UT26S 36651 284 84 5.2E-22 Sphingomonadales x
1 ABC transporter substrate-binding protein x gi|916882109 Roseivivax halodurans 38117 63 87 13 Burkholderiales
3 electron transfer flavoprotein subunit beta x x gi|544831343 Sphingobium baderi 26340 59 86 33 Sphingomonadales
6 MULTISPECIES: hypothetical protein x gi|490369075 Enterobacteriaceae 10525 70 87 2.9 Enterobacteriales
7 Elongation Factor Tu, partial x x gi|399117417 Taylorella asinigenitalis 14/45 17172 61 87 11 Burkholderiales
8 glycine—tRNA ligase subunit beta x x gi|917070028 Sphingomonas astaxanthinifaciens 73368 86 87 220 Sphingomonadales
9 MULTISPECIES: glutamate dehydrogenase x x gi|757782327 Gammaproteobacteria 49024 66 87 27 Enterobacteriales
10 hypothetical protein x gi|504827049 Acidovorax sp. KKS102 11788 68 87 3.8 Burkholderiales
11 PKHD-type hydroxylase x gi|759428465 Sphingobium sp. Ant17 24354 79 87 12000 Sphingomonadales x
17 L-ascorbate-6-phosphate lactonase ulaG x gi|595625006 Klebsiella pneumoniae 30684/NJST258_2 41773 55 87 94 Enterobacteriales
18 transcriptional regulator x gi|565806306 Xanthomonas hortorum 30827 60 87 27 Xanthomonadales
20 DNA gyrase subunit A x gi|670491751 Sphingomonas sp. FUKUSWIS1 101085 64 87 12 Sphingomonadales
21 type I restriction modification DNA specificity domain protein x gi|805605932 Burkholderia pseudomallei MSHR1079 48418 67 87 5.5 Burkholderiales
23 transposase x gi|852159985 Klebsiella pneumoniae 8434 64 87 10 Enterobacteriales
24 protein disulfide-isomerase x gi|916789948 Bradyrhizobium elkanii 23273 72 87 72 Rhizobiales
25 plasmid partitioning protein RepB x gi|516731572 Rhizobium leguminosarum 35961 58 87 42 Rhizobiales
26 hypothetical protein SEEE6482_13432 x gi|396069711 Salmonella enterica 12520 51 87 220 Enterobacteriales
28 hypothetical protein M770_18930 x gi|532132637 Pseudomonas aeruginosa VRFPA03 16995 74 87 1.2 Pseudomonadales
29 MULTISPECIES: cysteine ABC transporter substrate-binding protein x gi|970415048 Enterobacter 29569 65 87 8.4 Enterobacteriales
31 hypothetical protein OO17_23135 x x gi|763444705 Rhodopseudomonas palustris 8507 81 87 0.24 Rhizobiales
33 MULTISPECIES: (Fe-S)-cluster assembly protein x gi|760372574 Pseudomonas 11704 57 87 51 Pseudomonadales