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. 2017 Aug 28;13(8):e1006968. doi: 10.1371/journal.pgen.1006968

Fig 6. Kek-6 functions downstream of DNT2.

Fig 6

Confocal images of NMJs from A3-4 muscle 6/7 (left), and box-plot graphs (right), showing: (A) Over-expression of kek-6 in motoneurons rescued the phenotypes of kek-6 mutants. Dlg: One Way ANOVA p<0.0001 and post-hoc Bonferroni *p<0.05, ***p<0.001. HRP: One Way ANOVA p<0.001 and post-hoc Bonferroni **p<0.01, **p<0.01. (B) Left: Over-expression of kek-6 in neurons rescued the phenotype of DNT2 mutants. Dlg: Kruskal-Wallis p = 0.001, and post-hoc Dunn test **p<0.01, ***p<0.001. Right: kek-6 loss of function rescued the increase in boutons caused by the muscle over-expression of DNT2. Dlg: Welch ANOVA p<0.01 and post-hoc Games-Howell *p<0.05, **p<0.01. (C) Over-expression of kek-6 in motoneurons rescued the phenotypes of kek-6 DNT-2 double mutants. Dlg: Kruskal-Wallis p = 0.001 and post-hoc Dunn’s test *p<0.05, **p<0.01. HRP: Welch ANOVA p = 0.000, post-hoc Games Howell **p<0.01. n = 29–101 hemisegments. See S1 Table. GAL4 drivers: Muscle: MhcGAL4; Neurons: elavGAL4; MN: D42 or Toll-7GAL4. Controls: white boxes: yw/+; grey boxes: GAL4/+; mutant genotypes as in Fig 4. Rescue genotypes: (A) w; UASkek6RFP/+; Df(3R)6361/kek634D42GAL4. (B) w; UASkek6RFP/+; elavGAL4 Df(3L)6092/ DNT237; and w; UASDNT2-FL/+; Df(3R)6361/kek634D42GAL4. (C) w; Toll-7GAL4/UASkek6RFP; kek634Df(3L)6092/ Df(3R)6361 DNT237.