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. 2017 Aug 28;13(8):e1006968. doi: 10.1371/journal.pgen.1006968

Fig 7. Kek-6 and Toll-6 interact for NMJ structural homeostasis.

Fig 7

(A) Toll-6GAL4>mCD8-GFP is distributed in FasII+ motoneuron axons (arrows) at the muscle 6/7 NMJ terminal. (B) Muscle 6/7 NMJs (left) and box-plot graphs (right) showing: Toll-6MIO2127/Df(3L)BSC578 mutants had fewer 1b boutons. Toll-6–/–and Toll-6MIO2127Df(3R)6361/kek635 Df(3L)BSC578 double mutants had smaller NMJs (HRP, Kruskal-Wallis p = 0.0001) with reduced branching, and reduced active zones (Brp, Kruskal-Wallis p = 0.0055), post-hoc Dunn for both *p<0.05, ***p<0.001. Pre-synaptic over-expression of kek-6 in motoneurons in Toll-6-/-mutants (w; UASkek-6/+; Toll-6MIO2127GAL4/ Df(3L)BSC578) did not rescue NMJ size, but upregulated Brp+. Over-expression of activated Toll-6CY did not affect NMJ size (HRP) but increased active zones. N = 34–46 hemisegments. (C) Co-immunoprecitation from co-transfected S2 cells: Precipitating Toll-6 and Toll-7 with anti-Flag brought down Kek-6 detected with anti-HA. IP: immuno-precipitation; WB: western blot; asterisk: co-IP. See S1 Table. MN = D42GAL4.