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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Br J Haematol. 2017 Jun 23;178(6):914–926. doi: 10.1111/bjh.14801

Figure 3. The GO-203/LEN combination induces cell death by a ROS-dependent mechanism.

Figure 3

MM.1S and RPMI8226 cells were left untreated (CTL) and treated with (i) 2.5 μM GO-203 alone each day for 72 h, (ii) 2 μM lenalidomide (LEN) alone for 72 h, and (iii) GO-203 combined with LEN for 72 h. GO-203/LEN-treated cells were also incubated in the presence of 5 mM GSH for 72 h. A and B. Lysates were immunoblotted (IB) with the indicated antibodies. FL, full length. CF, cleaved fragment. C and D. The indicated cells were incubated with propidium iodide (PI)/annexin V and analysed by flow cytometry (left). The percentage of PI and/or annexin V positive cells is included in the panels (left). The results are expressed as the percentage (mean±SD of 3 determinations) of apoptotic/necrotic cells (right). E. The indicated cells were incubated with PI/annexin V and analysed by flow cytometry. The percentage of PI and/or annexin V positive cells is included in the panels. The results are representative of 3 independent experiments. * p<0.01.