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. Author manuscript; available in PMC: 2018 Sep 7.
Published in final edited form as: Cell. 2017 Aug 10;170(6):1120–1133.e17. doi: 10.1016/j.cell.2017.07.024

Figure 2. Identification of checkpoint blockade responsive B16BL6 tumor infiltrating T cellpopulations.

Figure 2

(A) Density t-SNE plots of an equal number of CD3ε+ B16BL6 tumor infiltrating T cells from eachtreatment group.

(B) t-SNE plot of infiltrating T cells overlaid with color-coded clusters.

(C) Plot of CD8/Treg ratios displayed on a per mouse basis with mean ± SD (*, P<0.05, unpaired T-test).

(D) t-SNE plot of tumor infiltrating T cells overlaid with the expression of selected markers.

(E) Frequency of T cell clusters displayed on a per mouse basis with mean ± SD (*, control v.s. anti- CTLA-4; #, control v.s. anti-PD-1; p<0.05, Dunnett's multiple comparison).

(F) Heat map displaying normalized marker expression of each T cell cluster.

Representative data from three independent experiments is shown.

See also Figure S2.