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. Author manuscript; available in PMC: 2018 Sep 7.
Published in final edited form as: Cell Stem Cell. 2017 Aug 10;21(3):319–331.e8. doi: 10.1016/j.stem.2017.07.009

Figure 5. TREX1-deficient astrocytes upregulate interferon and interferon-stimulated genes (see also Figure S6).

Figure 5

A, Expression of IFNα13 and IFNβ1 determined by qPCR (n = 3 cell lines, RTi, n = 1 cell line, NVP, n = 1 cell line). B, Expression of IFNα13 and IFNβ1 determined by qPCR in WT astrocytes transfected with ssDNA (n = 3 replicates). C, Western blot on astrocyte protein lysates measuring levels of phosphorylated IRF3, IRF3, and β-Actin. D, Quantification of phosphorylated IRF3/total IRF3 from western blot using protein lysates from isogenic cell lines and their treatments, normalized to V63fs (n = 4; 2 cell lines, with 2 technical replicates; RTi, n = 3, 2 cell lines with technical replicate; NVP, n = 3; 2 cell lines with technical replicate). E, Expression of ISGs determined by qPCR. The presented values are the means ± standard deviation (n = 3 cell lines; RTi, n = 3 cell lines; NVP, n = 1 cell lines). Student’s t-tests were performed to compare genotypes. Expression was normalized to zero, and B2M and HPRT1 were used as dual internal references. *P<0.05; **P<0.01; ***P<0.001.