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. 2017 Sep 8;7:11010. doi: 10.1038/s41598-017-11518-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PA induces lung oedema and epithelium damage during pneumonia. (a) Pulmonary bacteria loads of mice infected with PA for 24 and 48 hours. Data are representative of two independent experiments (n = 8). (b) Lung histological analysis from sham Swiss mice (panel 1) or PA infected mice at 6 (panel 2), 24 (panel 3), and 48hrs (panel 4) of infection. Magnification × 100 (bar = 100 μm). Data are representative of two independent experiments (n = 3). (c) Alveolar space quantification by SIOX analysis of histology slides presented in (b) (3 mice per slide. 4 fields per slide). (d) Red blood cell counts in BALF fluid in sham mice versus 24-hour infected mice. Data are representative of two independent experiments (n = 3). **p < 0.01 and ***p < 0.001.