Sizing nascent
SV40 DNA chains radiolabeled in the RNA moiety and crosslinked to
RPA subunits. (A) SDS–PAGE pattern of
photolabeled RPA subunits crosslinked to nascent SV40 DNA pulse-labeled
with NAB-dUTP and [α-32P]UTP.
(B) Size distribution of the DNA chains released
by proteinase K from the photolabeled RPA subunits indicated in
(A). (C) Densitometric profiles of (B), lanes 1
(red) and 2 (cyan). kDa, protein size markers; nt, nucleotide size
markers; Prcl, pre-clearing immuno-precipitate; Ipc, specific immunoprecipitate.