Figure 3.
Sizing nascent SV40 DNA chains radiolabeled in the RNA moiety and crosslinked to RPA subunits. (A) SDS–PAGE pattern of photolabeled RPA subunits crosslinked to nascent SV40 DNA pulse-labeled with NAB-dUTP and [α-32P]UTP. (B) Size distribution of the DNA chains released by proteinase K from the photolabeled RPA subunits indicated in (A). (C) Densitometric profiles of (B), lanes 1 (red) and 2 (cyan). kDa, protein size markers; nt, nucleotide size markers; Prcl, pre-clearing immuno-precipitate; Ipc, specific immunoprecipitate.