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. 2001 Sep 15;29(18):3892–3899. doi: 10.1093/nar/29.18.3892

Figure 6.

Figure 6

Change in Rpa1/Rpa2 photolabeling ratio during RDP elongation and further processing. Replicating SV40 chromosomes were pulse-labeled with BrdUTP and [α-32P]dATP in the absence of UTP. (A) Size distributions of nascent DNA chains at different incubation times. (B) Densitometric tracing of nascent DNA populations of the incubation times shown in (A). (C) Separation of RPA subunits photolabeled at the indicated incubation times. (D) Rpa1/Rpa2 photolabeling ratios at incubation times shown in (C).