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. 2017 Sep 8;7:10981. doi: 10.1038/s41598-017-11496-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparative eicosanoid catabolism profile in BMDMs and PMs stimulated with microbial particles. BMDMs and PMs (1 × 10⁶ cells/well) were adhered on cell culture plates for 24 h. Macrophages were stimulated with LPS (500 ng/mL) or zymosan (30 particles/cell) for 6 and 1.5 h, respectively. The lipid mediators in cell culture supernatants were identified and quantified by HPLC-MS/MS (MRM mode) for eicosanoids: TXB₂, PGD₂, PGE₂, PGJ₂/PGA₂, 6-keto-PGF_1α, 12-HETE, 15-HETE, 5-HETE, 5-oxo-ETE, LTC₄, LTB₄, and Δ-6-trans-LTB₄ as well as for the release of fatty acids: AA and DHA. Results are expressed as the means ± s.e.m. of three experiments (n = 3). Differences were considered when p < 0.05, *BMDMs and PMs stimulated or not, compared to non-stimulated basal BMDM production (dashed line), ^#PM compared to BMDM eicosanoid production, after LPS or zymosan stimulation.