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. 2017 Sep 8;7:10981. doi: 10.1038/s41598-017-11496-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of BMDM polarization on eicosanoids production after microbial particle stimulation. BMDMs were treated with IFN-γ (100 ng/mL) (M1), IL-4 + IL-13 (10 ng/mL) (M2), or only adhered (M0). Then, BMDMs were stimulated with LPS (500 ng/mL) or zymosan (30 particles/cell) for 6 and 1.5 h, respectively. The lipid mediators in cell culture supernatants were identified and quantified by HPLC-MS/MS (MRM mode) for eicosanoids: TXB₂, PGD₂, PGE₂, PGJ₂/PGA₂, 6-keto-PGF_1α, 12-HETE, 15-HETE, 5-HETE, 5-oxo-ETE, LTC₄, LTB₄, and Δ-6-trans-LTB₄, as well as for the release of fatty acids: AA and DHA. The absence of specific eicosanoid production is represented by a red square (N.D.). Results are expressed as the means ± s.e.m. of three experiments (n = 3). Differences were considered significant when p < 0.05, *M1 and M2 compared to M0 and ^#M2 compare to M1.