Table 2.
Comparative enzymatic constants of Vp16 PDF and chimeras using Fo-Met-Ala-Ser as substrate.
| PDF enzyme | k cat (s−1) | K m (mM) | k cat /K m (M−1.s−1) | Relative k cat/K m (%) |
|---|---|---|---|---|
| Vp16 PDF1B | 20 ± 1 | 2.3 ± 0.9 | 8,478 ± 2276 | 100 |
| Vp16 PDF(KLF)helices | nm | nm | nm | nm |
| Vp16 PDF(VTI)helices | 3.17 | 1 | 7,417 ± 6006 | 92 |
| Vp16 PDF(VTF)helices | 0.4 | 0.5 | 80 | 18 |
| E. coli PDF1B | 144 | 3.8 | 53,846 ± 21,667 | 100 |
| E. coli PDF(KLF)Δhelices | 77 ± 9.4 | 5.9 ± 2.1 | 13,125 ± 4,560 | 66 |
| E. coli PDF(VTI)helices | 26 ± 20 | 1.6 ± 1.3 | 19,316 ± 9,916 | 97 |
| E. coli PDF(VTI)Δhelices | ND | ND | ND | ND |
| E. coli PDF(KLI)helices | 41 ± 0.4 | 3.1 ± 0.11 | 13,189 ± 3,515 | 67 |
nm, not measurable.
ND, not determined.