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. 2017 Sep 8;7:10941. doi: 10.1038/s41598-017-11287-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Loss of Nrp1 in basal cells compromises mammary development. (A) Representative images of whole mount carmine staining for 7-week old K14-Cre, K14-Cre;Nrp1 ^fl/+ and K14-Cre;Nrp1 ^fl/fl mice. Dash line, forefront of epithelium extension; dash line circle, lymph node (L.N.). Scale bar, top panels, 2mm; bottom panels, 2mm. (B) Quantification of the mammary duct extension in K14-Cre, K14-Cre;Nrp1 ^fl/+ and K14-Cre;Nrp1 ^fl/fl mice. The distance from L.N. to epithelium forefront was measured. n = 3 mice in each group. Data are presented as mean ± SEM. ***P < 0.0001. (C) qPCR analysis validating the Nrp1 knockout efficiency using FACS-isolated basal cells. (D) Whole mount imaging of mammary glands with lineage-traced mGFP at 6-week old. (E,F) FACS analysis indicating the proportion of basal and luminal cells, and the percentages of mG+ cells in basal and luminal compartments (E). Quantification indicating no significant differences of mG+ cell percentage in either basal or luminal compartment when comparing the Nrp1 knockout (fl/fl) and the Ctrl (fl/+) (F). n = 3 mice in each group.