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. 2017 Sep 8;7:10972. doi: 10.1038/s41598-017-11592-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Metabolomics of silkworm larvae reared on fresh mulberry leaves or the artificial diet. (a) Partial least squares-discriminant analysis (PLS-DA). (b) Classification of metabolites and (c) the heat map. Data from the PLS-DA were analyzed using SIMCA-P 13.0 software (Umetrics AB, Umeå, Sweden). The x-axis shows the names of the metabolites, and the y-axis shows the names of the samples (strain-food-sex). Hi and Lo indicate silkworm strains that exhibited high or low ingestion of the artificial diet, respectively. M, fresh mulberry leaves, n = 8 replicates; A, artificial diet, n = 7 replicates; ♂, male; ♀, female. Each sample comprised five larvae, which provided an equal volume of hemolymph.