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. 2017 Sep 8;7:10972. doi: 10.1038/s41598-017-11592-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Abundance plot of key metabolites in the hemolymph of silkworms reared on the artificial diet or mulberry leaves. (a) Main vitamins, (b) main metabolites in the urea cycle, and (c) major constituent amino acids in silk protein and some related amino acids. Data were derived from metabolomic assay data, and samples marked with the same letter did not differ significantly from each other (P > 0.05). Lowercase (a,b and c) and capital letters (A, B and C) indicate a significant difference between the female silkworm samples and male samples, respectively. Hi and Lo indicate silkworm strains that exhibited high or low ingestion of the artificial diet, respectively. M, fresh mulberry leaves, n = 8 replicates; A, artificial diet, n = 7 replicates; ♂, male; ♀, female.