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. 2017 Sep 8;7:10972. doi: 10.1038/s41598-017-11592-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Heat map analysis of differential metabolic pathways in silkworms reared on the artificial diet or mulberry leaves. The Hi silkworm strain was used for the comparison between genders. The analysis was based on a visual analysis of metabolic pathways⁶². A pathway activity profiling (PAPi) algorithm was used to calculate the activity score (AS) for each metabolic pathway, and the calculated PAPi and the relative abundance of each metabolic pathway were based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. The metabolic pathway heat map was produced based on the KEGG metabolic process classification, and an analysis of variance was used for the statistical test (P < 0.05 indicates significance). Different circles represent the AS values of different metabolic pathways.