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. 2017 Sep 8;7:10972. doi: 10.1038/s41598-017-11592-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of differential metabolites between genders. (a) The number of differential metabolites between genders in different silkworm strains that were fed different diets. (b) Metabolites with stable differences (P < 0.05) between genders in both silkworm strains reared on the two diets. (c) Metabolites exhibiting significant differences in abundance between the hemolymph of silkworm genders reared on mulberry leaves (P < 0.05), but without significant differences between genders in the silkworms reared on the artificial diet, and metabolites exhibiting significant differences in abundance between the genders in the silkworms reared on the artificial diet, but with opposite patterns compared with those reared on mulberry leaves. (d) Metabolites with significant differences in abundance in the hemolymph between genders of the silkworms reared on the artificial diet (P < 0.05), but without significant differences between genders in the silkworms reared on mulberry leaves (P ≥ 0.05). FC indicates the fold-change of the relative abundance (male/female). No fill color indicates that there was no significant difference in metabolite abundance between males and females.