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. 2017 Sep 8;7:11028. doi: 10.1038/s41598-017-11403-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Blocking macrophages prevents outgrowth of PyMT tumour cells. (a) Experimental design used to block macrophages during transplantation. (b) Plug mass 3 weeks after PyMT transplantation in presence in either PBS or Clodronate (Clod) liposomes. *P value < 0.05 (n = 7, two tail T-test). (c) H&E (Upper panels) and immunohistochemical staining for F4/80 (Lower panels) 1 week post-transplantation in growth factor reduced matrigel with either PBS or Clodronate. Scale bar: 50 µm. (d,e) H&E of staining of PBS or Clodronate treated plugs at 3-week post-transplantation. Scale bar: 200 µm (d); Scale bar: 50 µm (e). (f) Immunohistochemical staining for αSMA of PBS or Clodronate treated plugs 1 week post-transplantation. Scale bar: 50 µm.