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. 2017 Sep 8;7:11028. doi: 10.1038/s41598-017-11403-w

Figure 5.

Figure 5

PyMT cells in clodronate treated Matrigel retain intrinsic ability to form tumour. (a) Experimental design for primary transplant, in presence of either PBS or Clodronate liposomes. (b) After 3 weeks secondary transplants in naïve mice. (c) Plug mass of primary transplant 3 weeks post-transplantation in growth factor reduced matrigel. (PBS n = 2, Clodronate n = 6). (d) H&E of PBS or Clodronate treated plugs at 3 weeks post-transplantation. Scale bar: 100 µm. Inset: higher magnification. Scale bar: 100 µm. (e) Plug mass of secondary transplants 2.5 weeks post-transplantation in Matrigel only. (n = 4, n.s. two tail T-test non-significant). (f) H&E of PBS or Clodronate treated plugs at 2.5 weeks post-transplantation. Scale bar: 100 µm. Inset: higher magnification. Scale bar: 100 µm.