Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 8;7:11000. doi: 10.1038/s41598-017-11374-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Expression of MIER3 mRNA and protein in CRC cells and colorectal tumor tissue samples. (A) Expression of MIER3 protein by western blotting (above). The protein expression levels were quantified by comparing the gray level of each band using Quantity One Software (below). Data are the means ± SD from 3 parallel experiments. (B) Expression of MIER3 mRNA by real-time PCR in six CRC cell lines. Expression levels of MIER3 mRNA were normalized with β-actin mRNA levels. Error bars represent the mean ± SD calculated from 3 parallel experiments. (C) Expression of MIER3 protein in each of the primary CRC (T) and adjacent noncancerous tissues (N) paired from the same patient by western blotting (left) (n = 12). The protein expression levels were quantified by comparing the gray level of each band using Quantity One Software (right). Error bars represent the mean ± SD calculated from 3 parallel experiments. (D) Expression levels of MIER3 mRNA by real-time PCR in paired CRC and adjacent normal tissues (n = 32). Expression levels of MIER3 mRNA were normalized with β-actin mRNA levels. Error bars represent the mean ± SD calculated from 3 parallel experiments. (E) MIER3 expression in CRC tissues with or without metastases. nmCRC denotes CRC tissues without metastases; mCRC denotes CRC tissues with lymph node metastases.