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. 2017 Sep 8;7:11000. doi: 10.1038/s41598-017-11374-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MIER3 is associated with Sp1 and promotes EMT in CRC cells. (A) MIER3 co-immunoprecipitates with Sp1 in CRC cells. Lysates from SW620 cells were immunoprecipitated with MIER3 antibody or control IgG and detected with Sp1 antibody on a western blot and then immunoprecipitated with Sp1 antibody or control IgG and detected with MIER3 antibody on a western blot. (B) The spindle cell phenotype of SW620/shMIE3 cells and the epithelial phenotype of CRC cells from the SW620/shNC control group showing epithelial-to-mesenchymal transition (EMT) induced by decreased MIER3 expression. (C) MIER3 knockdown led to increased Sp1 expression and induced hallmarks of EMT, including decreased E-cadherin and ZO-1 and the accumulation of vimentin and N-cadherin in CRC cells. In contrast, down-regulation of Sp1 led to decreased E-cadherin and ZO-1 expression and increased vimentin and N-cadherin expression. (D) MIER3 overexpression led to decreased Sp1 expression and induced the accumulation of E-cadherin, ZO-1 and the decreased expression of vimentin, and N-cadherin in CRC cells. While up-regulation of Sp1 led to increased E-cadherin and ZO-1, it inhibited vimentin and N-cadherin expression. Error bars represent the mean ± SD.