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. 2017 Sep 8;7:10948. doi: 10.1038/s41598-017-11253-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dynamics of intracellular calcium and reactive oxygen species (ROS) in hTDCs cultured under short-term magnetic stimulation. (a–d) Detection of intracellular calcium (green) determined using the fluorescent dye Fluo-3 AM. Scale bar, 20 µm. (e,f) Quantification of fluorescence signal of intracellular calcium (cyt Ca²⁺). A minimum of 35 cells were analysed per condition and data are expressed as scattered dot plot with mean. (g,h) ROS determination according to the DCFDA assay and results are expressed as mean ± SEM (n = 9 experimental replicates from 3 biological replicates).