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. 2017 Sep 8;7:11030. doi: 10.1038/s41598-017-10704-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Two-stage lysis. (a) Diagram of the process flow: a single cell is trapped and its membrane is lysed releasing the mRNA that is collected. Next, the DNA is stained with fluorescent YOYO-1 dye to confirm its presence in the trap before it is lysed and genomic DNA can be collected in the outlet. (b) Bright field and fluorescence and merged time-lapse images from the instrument, during trapping, cell membrane lysis (loss of cytosol dye) and staining of the nuclear DNA retained in the cell trap. The scale bar corresponds to 15 μm. The white arrow in bright-field image shows the position of the trapped cell. In the merged image the fluorescence is shown in colour.