. 2017 Sep 8;7:11039. doi: 10.1038/s41598-017-10445-4

Table 1.

Primer sequences.

Gene	Primer Sequence	Primer Type	Tm (°C)
DEFB1	Reverse: cgccGGTAGGAAGTTCTCATGGcG	Probes Master	60
DEFB1	Forward: GTCAGCTCAGCCTCCAAAGGA	Probes Master	60
Defb4	Forward: GTGAAGCTCCCAGCCATCAG	SYBR Green I Master	58
Defb4	Reverse: GATTGCGTATCTTTGGACACC	SYBR Green I Master	58
DEFB4	SA Biosciences commercial primer	SYBR Green I Master	60
DEFA5	Forward: GCCATCCTTGCTGCCATTC	SYBR Green I Master	58
DEFA5	Reverse: GATTTCACACACCCCGGAGA	SYBR Green I Master	58
hCAP-18	Forward: cgctGACGGGCTGGTGAAGcG	Probes Master	55
hCAP-18	Reverse: CCCAGCAGGGCAAATCTCTT	Probes Master	55
TLR5	Forward: CAGAGACTGGTGTTCAAGGAC	SYBR Green I Master	54
TLR5	Reverse: GTGTCCAGGTGTTTGAGCA	SYBR Green I Master	54
GAPDH	Primer Design commercial primer	SYBR Green I Master	60
GAPDH	(House-keeping reference gene)	SYBR Green I Master	60
ATP5B	Primer Design commercial primer	SYBR Green I Master	60
ATP5B	(House-keeping reference gene)	SYBR Green I Master	60
Mouse β-actin	Forward: TGAGAGGGAAATCGTGCGTGACAT	SYBR Green I Master	60
Mouse β-actin	Reverse: ACCGCTCATTGACGATAGTGATGA	SYBR Green I Master	60