Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 8;7:11006. doi: 10.1038/s41598-017-11212-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Loss of Gal-1 suppresses methionine- and choline-deficient (MCD) diet-induced liver fibrosis. (A,B) MCD diet-induced collagen deposition is reduced in Gal-1 null mice (Gal-1-KO) (n = 6) compared to wild-type mice (WT) (n = 6). Liver fibrosis was induced by feeding mice with an MCD diet for 8 weeks. Collagen was detected using picrosirius red staining. The left panel shows the representative images. The right panel shows the quantitative data of picrosirius red staining using ImageJ software. (C,D) HSC activation is reduced in Gal-1 null mice compared to wild-type mice. HSC activation was examined by measuring α-smooth muscle actin (α-SMA) expression using Western blotting (left panels) which confirmed that loss of Gal-1 suppresses HSC activation and extracellular matrix production in livers of MCD diet-fed mice. (E) Serum AST and ALT amount are similar between Gal-1 null mice and wild-type mice. Serum AST and ALT were measured using a VetTest® Chemistry Analyzer. (F) Loss of Gal-1 does not change the RNA expression levels of proinflammatory cytokines (IL-1β, TNF-α, CCL2) compared to wild-type mice. The RNA was extracted from mouse livers and was converted to cDNA. Proinflammatory cytokines expression was measured using RT-qPCR. Relative expression levels were calculated by comparing ∆CT values of each group to those of wild-type mice without treatment. Data are shown as folds of change.