Figure 4.

In situ DNA polymerase activity of Li Pol β. Autoradiography of the in situ gel analysis carried out as described in Materials and Methods. The activity was assayed in the presence of Mg²⁺ (A) and Mn²⁺ (B) as divalent metal ions. The activity was measured in complete extracts from non-induced (–) and IPTG induced (IPTG) pQE32 E.coli strain as well as in the recombinant purified fraction (Ni²⁺-NTA). The activity bands corresponding to the endogenous activities of E.coli: Pol I and its Klenow fragment are marked as well as the corresponding to Li Pol β. The putative 35 kDa Li Pol β active fragment is indicated by a question mark.