Figure 7.

Nuclear localization of Li Pol β by immunofluorescence. Leishmania infantum promastigotes were recovered from asynchronous cultures at the logarithmic and stationary phases. The DNA staining with DAPI shows the nucleus (n) and kinetoplast (k) localization at the promastigote (blue). Li Pol β and Li Topoisomerase II are localized by the respective specific antibodies and anti-rabbit IgG-FITC (green). (A) Li Pol β at logarithmic phase promastigotes. (B) Li Pol β at stationary phase promastigotes. (C) Li Topoisomerase II at logarithmic phase promastigotes. Bar 2 µm. For further experimental details see Materials and Methods.