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. 2017 Sep 8;7:11051. doi: 10.1038/s41598-017-11428-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Longitudinal nodule sections of Lupinus albus coinoculated with Bradyrhizobium sp. CAR08 and Micromonospora ML01-gfp (21 dpi). (a) Green fluorescence signal captured by CLSM of infected cells containing Micromonospora ML01-gfp. (b) Overlay of light and fluorescence images of the nodule section. (c) Green fluorescence localization of ML01-gfp in a nodule section stained with propidium iodide and viewed by CLSM. (d) Higher magnification image captured with the green channel. (e) Higher magnification image captured with the red channel. (f) Composite image of both channels. The white rectangle in image c shows the area where images d-f were captured. C, cortex; BZ, bacteroid zone; dpi, days post inoculation. Bars: 100 µm (a, b, c); 40 µm (d, e, f).