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. 2017 Sep 8;7:11051. doi: 10.1038/s41598-017-11428-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Trifolium longitudinal nodule section (20 dpi) showing the distribution of infected cells after co-inoculation with Micromonospora ML01-gfp and Rhizobium E11-mCh captured by CLSM. (a) Image obtained with the green channel for the localization of Micromonospora. The circled area indicates fluorescence emitted by Micromonospora concentrated in the bacteroid zone. (b) Image captured with the red channel for the localization of Rhizobium. (c) Combination of images a and b. (d) Detail of infected zone showing the co-localization of Micromonospora and Rhizobium in the host cells. The white circle in 6c shows the area where image (d) was captured. Bars: 60 µm (a–c); 20 µm (d). dpi, days post inoculation; C, cortex; BZ, bacteroid zone.