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. 2017 Sep 8;7:10990. doi: 10.1038/s41598-017-11134-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The dsRNA-mediated suppression of (A) TmELO1 and (B) TmELO2 transcrpts in mature larvae. 5000 ng dsRNA was injected in every larva. Students t test was used in data analysis. Y axis values are mean ± SE of relative transcript level. Uppercase letters (A–C) represent significant differences (P < 0.01) according to Tukey’s multiple range test. (C). Mature larvae of T. molitor were injected with dsRNA of EGFP or TmELO1 or TmELO2. There were three replicates and each replicate included 40 mature larvae. Y axis values are mean ± SE of the death rate. Uppercase letters (A,B) represent significant differences (P < 0.01) according to Tukey’s multiple range test.