Table 4.
Sequences of primers used in DNA cloning, dsRNA synthesis and qRT-PCR.
| Primer name | Primer sequence (5′-3′) | Purpose |
|---|---|---|
| TmELO1-F | CTGTGCGGAAAACGGAAGAG | cDNA cloning |
| TmELO1-R | ATTTCGCCCCTGAGTTAC | |
| TmELO2-F | AGTGACTTGTGTTCTGTG | |
| TmELO2-R | AATTGCATTACGCCCGAGG | |
| eTmELO1-F | CGGGATCCATGGCTCACCTAGTTACCAG | yeast expression |
| eTmELO1-R | CCCTCGAGCTATTTGATCTTCTTTGAG | |
| eTmELO2-F | CGGGATCCATGGCACAAATATTAAC | |
| eTmELO2-R | CCCTCGAGTTACGCCCGAGGGTTGTCT | |
| dsTmELO1-F1 | GATCACTAATACGACTCACTATAGGGGTGTCCAAACACTGTTCAG | dsRNA fragments |
| dsTmELO1-R1 | GATCACTAATACGACTCACTATAGGGATTGCCGCCACCATGTAA | |
| dsTmELO2-F1 | GATCACTAATACGACTCACTATAGGGCACGACCTCATGGATAAC | |
| dsTmELO2-R1 | GATCACTAATACGACTCACTATAGGGTGGAGGGTCGAAATGTGG | |
| dsTmELO1-F2 | GATCACTAATACGACTCACTATAGGGATGGCTCACCTAGTTACCAG | |
| dsTmELO1-R2 | GATCACTAATACGACTCACTATAGGGGTTGACACCTGAAGCTGT | |
| dsTmELO2-F2 | GATCACTAATACGACTCACTATAGGGCTAGATACGATCTTCTTCG | |
| dsTmELO2-R2 | GATCACTAATACGACTCACTATAGGGTAGACCTATCCACCAGAC | |
| dsEGFP-F | GATCACTAATACGACTCACTATAGGGATGGTGAGCAAGGGCGAGGAGC | |
| dsEGFP-R | GATCACTAATACGACTCACTATAGGGGATCACTAATACGACTCACTATAGGG | |
| qTmELO1-F | TACTCATCAGTTCCAGCTTCT | qRT-PCR |
| qTmELO1-R | CACCGTTATTACTACTCTTCACA | |
| qTmELO2-F | CACGCCGTCATGTTCTACT | |
| qTmELO2-R | ACGCCCGAGGGTTGTCTAT | |
| qTmRpS3-F | GTGGTCGTTTCTGGCAAACT | |
| qTmRpS3-R | CAACACTCCTTGCCTCAACA |
Xho I site (GGATCC), BamH I site (CTCGAG) and T7 promoter sequences (GATCACTAATACGACTCACTATAGGG) are underlined.