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. 2017 Sep 8;7:11045. doi: 10.1038/s41598-017-11643-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Reduced levels of α-granule proteins SPARC, VWF and PF4 during storage. (a) Non-imputed LFQ values of SPARC (blue) and SPARC levels as measured with ELISA (red). (b) Non-imputed LFQ values of VWF (blue) and VWF levels as measured with ELISA (red). (c) Western blot analysis of SPARC, LFQ values (blue) and intensities of Western blot (red). (d) Western blot analysis of VWF, LFQ values (blue) and intensities of Western blot (red). Calmodulin was used as loading control. Bands were normalized according to intensity of calmodulin (loading control), and average intensity per membrane was normalized to 1. (e) Non-imputed LFQ values of PF4 (blue) and PF4 levels as measured with ELISA (red). Data represents mean ± standard deviation (n = 3), *P < 0.05 compared to day 1 from this time point onwards. Uncropped immunoblots of SPARC, VWF and calmodulin can be found in Supplementary Data S5.