Skip to main content
. 2017 Sep 4;8:1076. doi: 10.3389/fimmu.2017.01076

Figure 2.

Figure 2

CCCTC-binding factor (CTCF) deficiency increases the rate of class switch recombination (CSR) in vitro. (A) Representative flow cytometry analysis of surface IgG1 expression 1 in splenic B cells from CTCFfl/+ and CTCFfl/fl mice after 48 h (top panels) and 72 h (bottom panels) of lipopolysaccharide (LPS) + IL-4 stimulation. (B) Quantification of IgG1 cells in CTCFfl/+ (n = 12) and CTCFfl/fl (n = 11) B cells. Each dot corresponds to a B cell culture from an individual mouse. Time of analysis is indicated underneath. Mean values are indicated in horizontal. p(48 h) < 0.0001; p(72 h) < 0.0001; p(96 h) = 0.0119. (C). Representative flow cytometry analysis (top) of surface expression of CSR in splenic B cells from CTCFfl/+ (n = 4) and CTCFfl/fl (n = 4) mice after 72 h of anti-CD40 + IL-4 (left), LPS (middle), and TGFβ + anti-CD40 + IL-4 (right) stimulation. Quantification of IgG 48 and 72 h after stimulation is shown on the bottom. p(anti-CD40 + IL-4) = 0.0022; p(LPS) = 0.0021; p(TGFβ + anti-CD40 + IL-4) = 0.033. Statistical analysis was done with two-tailed unpaired Student’s t-test.