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. 2017 Sep 4;8:1076. doi: 10.3389/fimmu.2017.01076

Figure 5.

Figure 5

CCCTC-binding factor (CTCF) deficiency enhances germline transcription (GLT) in resting B cells. (A,B) qRT-PCR analysis of germline transcript μ (A), γ-1 (B) in resting splenic B cells (0 h) or after lipopolysaccharide + IL-4 stimulation from CTCFfl/+ and CTCFfl/fl (n = 3–7). p(0 h, GLTγ1) < 0.0001. (C) qRT-PCR analysis of germline transcript γ-3 (left), γ-2b (right) in resting splenic B cells. p(0 h, GLTγ3) = 0.0353; p(0 h, GLTγ2b) = 0.0307. Data are shown as the ΔCt value calculated from the difference in the Ct of the GLT and GAPDH. Statistical analysis was done with two-tailed unpaired Student’s t-test.