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. 2017 Sep 4;8:1063. doi: 10.3389/fimmu.2017.01063

Figure 4.

Figure 4

TLR2 is involved in lactobacillus NK318.1-mediated STAT3 activation. (A) qRT-PCR and ELISA (lower) of IL-10 in the wild-type (WT) and TLR2−/− macrophages in response to heated-dead Lactobacillus 318.1. Macrophages were isolated from the ileum LP. (B) Immunoblotting of pp65, pSTAT3, pp-38, pJNK, and pERK in the isolated macrophages from the ileum LP of WT and TLR2−/− mice after exposing to Lactobacillus NK318.1. (C) Flow cytometry of MHCII(+)F4/80(+) and F4/80(+)IL-10(+) macrophages in the small intestinal (S.I) LP of WT and TLR2−/− mice with (L.NK.1) or without (NC) Lactobacillus NK318.1 gavage. The proportion of MHCII(+)F4/80(+) and F4/80(+)IL-10(+) macrophages in WT and TLR2−/− mice with (L.NK1) or without (NC) L.NK1 gavage were compared (right; n = 5). (D) Comparison of IL-10 in the ileum LP of WT and TLR2−/− mice with (L.NK.1) or without L.NK.1 (NC) gavage (n = 5). *p < 0.05, **p < 0.01, and ***p < 0.001 [t-test in (A); one-way analysis of variance in (C,D), mean ± SD]. NS, no significant; qRT-PCR, quantitative real-time PCR; pJNK, phosphor-JNK; pERK, phosphor-ERK; WT, wild-type; LP, lamina propria.