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. 2017 Sep 4;8:1063. doi: 10.3389/fimmu.2017.01063

Figure 5.

Figure 5

Lactobacillus NK318.1-mediated macrophages depend on STAT3. (A) Flow cytometry of F4/80(+)CD11b(+) and F4/80(+)CD11C(+) macrophages in the small intestinal (S.I) lamina propria (LP) of wild-type (WT) mice with (L.NK.1/StI) or without (L.NK.1) STAT3 inhibitor. F4/80(+)CD11b(+) and F4/80(+)CD11C(+) macrophage populations in the WT mice with (L.NK.1/StI) or without (L.NK.1) STAT3 were compared (right, n = 6). (B) Comparison of total F4/80(+)CD11b(+) macrophages in whole S.I LP of WT mice with (L.NK.1/StI) or without (L.NK.1) STAT3 inhibitor were compared (n = 6). (C) Flow cytometry of the macrophages in S.I LP of huREG3γtgIEC and control littermate WT mice. F4/80(+)CD206(+), F4/80(+)Gr-1(+), and F4/80(+)IL-10(+) macrophages in the LP were compared (right, n = 6). (D) Comparison of F4/80(+)CD11b(+), F4/80(+)CD11C(+), F4/80(+)CD206(+), and F4/80(+)Gr-1(+) macrophages in the Peyer’ s patches (PP) and spleen of WT mice with (L.NK1/StI) or without (L.NK.1) STAT3 inhibitor were compared (n = 6). (E) Comparison of F4/80(+)IL-10(+) macrophages in the PP and spleen of WT mice with (L.NK1/StI) or without (L.NK.1) STAT3 inhibitor were compared (n = 6). (F) Immunoblotting of phosphor-STAT3 in the ileum tissues of WT mice with (L.NK1/StI) or without (L.NK.1) STAT3 inhibitor (number, different individuals). (G) IL-10 ELISA of ileum tissues in WT mice with (L.NK1/STAT3i) or without (L.NK1) STAT3 inhibitor (n = 6). NC, mice without Lactobacillus gavage and STAT3 inhibitor injection; L.NK.1, mice with Lactobacillus gavage but without injecting STAT3 inhibitor; L.NK.1/StI, mice with Lactobacillus gavage and STAT3 inhibitor injection. *p < 0.05, **p < 0.01, and ***p < 0.001 (one-way analysis of variance, mean ± SD). NS, no significance. Data for all panels are a representative from two to three experiments.