Figure 6.

Accumulation of interleukin (IL)-10(+) macrophages in adipose tissues of huREG3γ^tgIEC mice that are resistant to high-fat diet-mediated obesity. (A) Body weights of huREG3γ^tgIEC and control littermate wild-type (WT) mice after high-fat diet. huREG3γ^tgIEC (REG3g) and control littermate WT mice (n = 12, male) were observed and weighed at the indicated times after giving HFD. These mice are no differences at baseline before high-fat feeding. (B,C) Comparison of fat pad tissues of WT and huREG3γ^tgIEC. The images in (C) were a representative from 12 mice. (D,E) Glucose (D) and insulin (E) tolerance in male huREG3γ^tgIEC (REG3g) and control littermate WT mice after high-fat diet. WT and huREG3γ^tgIEC (n = 6, male) mice after HFD for 3 months were analyzed for glucose and insulin tolerance. (F,G) Body (F) and fat pad (G) weights of mice with (L/NK.1) or without (NC) Lactobacillus NK318.1 gavage (1 × 10⁹; once per week) during high-fat diet. (H,I) Glucose (H) and insulin (I) tolerance in mice with (L/NK.1) or without (NC). (J) Flow cytometry of macrophages in the adipose of huREG3γ^tgIEC (REG3g) and control littermate WT mice. CD11C(+)F4/80(+) and F4/80(+)IL-10(+) cells in the adipose tissues were compared (right, n = 6). (K) Comparison of CD11C(+)F4/80(+) and F4/80(+)IL-10(+) cells in the adipose tissues of mice with (WT/L.NK.1) or without (WT/NC) Lactobacillus gavage (right, n = 6). (L) Immunostaining of F4/80(+) macrophages in the adipose of the huREG3γ^tgIEC (REG3g) and control littermate WT mice. *p < 0.05, **p < 0.01, and ***p < 0.001: one-way analysis of variance in (A,D,E,F,H,I); t-test in (B,G,J,K). Data are a representative of three independent experiments.