Skip to main content
. 2017 Sep 6;19(10):830–841. doi: 10.1016/j.neo.2017.08.002

Figure 2.

Figure 2

The role of p53 and p21 in SCH900776-mediated sensitization of HCT116 cells to LA-12/cisplatin-induced cytotoxicity. (A, B) Total and phosphorylated (Ser15) p53, total p21, cleaved PARP, γ-H2AX, phosphorylated (Ser296), and total Chk1 level (Western blotting) in wt versus p53−/− or wt versus p21−/− HCT116 cells pretreated (2 hours) with SCH900776 (SCH, 3 μM) and subsequently treated (48 hours) with LA-12 (0.75 μM) or cisplatin (CDDP, 20 μM). (C) Percentage of annexin V+/PI− or (D) annexin V+/PI+ (flow cytometry) wt, p53−/−, and p21−/− HCT116 cells treated as in A and B. Results are means ± SEM or representatives of three independent experiments. Statistical significance: P < .05, * versus control, ‡ versus SCH, Ο versus LA-12 or CDDP and Δ for wt versus p21−/− or wt versus p53−/− cells.