Supplementary Figure 2.

(A) Cleavage of PARP, caspase-9, caspase-3, and Chk1 level (Western blotting), and (B) cleavage of caspase-3 (flow cytometry) in HCT116 wt cells transfected (24 hours) with control or Chk1 siRNA pretreated (2 hours) with SCH900776 (SCH, 3 μM) and subsequently treated (72 hours) with LA-12 (0.75 μM) or cisplatin (CDDP, 20 μM). Results are means ± SEM or representatives of three independent experiments. Statistical significance: P < .05, * versus control, ‡ versus SCH, Ο versus LA-12 or CDDP and Δ for control versus Chk1 siRNA.