Table-2.

The physical, chemical, and secondary structure predictions for the cattle β-defensins.

Name	Physiochemical parameters						Secondary structure predictions
	Amino acid length	Molecular weight	Theoretical PI	Instability index	Aliphatic index	GRAVY*	α helix	β helix	Transmembrane helix
TAP	64	6953.1	10.26	18.54	103.44	0.431	33	30	25
LAP	64	7041.50	11.24	50.66	95.94	0.122	47	16	25
DEFB1S1	60	6665.98	8.98	28.16	102.33	0.447	48	18	-
DEFB1S2	54	6115.0	9.30	76.97	77.59	-0.519	46	11	-
DEFB3	60	6764.17	11.47	18.00	97.50	0.243	23	33	-
DEFB4	63	7233.74	11.46	46.73	86.51	0.278	33	25	-
DEFB5	64	7227.73	10.47	59.02	95.78	0.380	53	9	25
DEFB10	62	6954.46	10.35	39.25	114.68	0.429	50	13	-
DEFB103	67	7614.31	9.93	40.62	106.27	0.185	52	18	24

TAP=Tracheal antimicrobial peptide, LAP=Lingual antimicrobial peptide, DEFB1S1 and DEFB1S2=Splice variants of β-defensin 1 peptide, DEFB3=β-defensin 3 peptide, DEFB4=β-defensin 4 peptide, DEFB5=β-defensin 5 peptide, DEFB10=β-defensin 10 peptide, DEFB103=β-defensin 103 peptide,

^*GRAVY=Grand average of hydropathicity, PI=Isoelectric point