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Evaluation of microvascular endothelial function by intravital fluorescence microscopy. Percentage of variation from the basal values of arteriolar inner diameter after topical acetylcholine (Ach, 10−6 μM) administration in the cranial window of rats fed a normal or high-fat diet (CTL-sham, HFD-sham) after cerebral ischemia and reperfusion (CTL-IR or HFD-IR). Values represent the mean ± SEM. n = 4 per group. *p < 0.05, ***p < 0.001 versus CTL-sham, #p < 0.05 versus CTL-IR
