Table 1.
Summary of genomic DNA (gDNA) validation of novel alleles predicted by bulk repertoire sequencing data.
| Novel alleles |
||||||
|---|---|---|---|---|---|---|
| Subjects | IGHV1-8*02 (G234T) | IGHV1-69*01 (G163A) | IGHV3-30*02 (T201C) | IGHV4-31*02 (C198T) | IGHV4-59*01 (T109C) | IGHV4-61*01 (C93T_C136G_A138C) |
| 1 | +/+ | +/+ | +/+a | −/− | +/+ | −/− |
| 2 | −/N.D. | −/− | −/− | +/+ | −/− | −/−b |
| 3 | −/− | +/+a | −/− | −/− | +/+ | +/+ |
| 4 | +/+ | −/− | −/−a | −/− | −/− | −/− |
| 5 | −/− | −/− | −/− | +/+ | −/− | −/− |
| 6 | +/+ | −/−a | +/+ | −/−b | +/+ | −/−b |
| 7 | +/+ | −/− | +/+a | −/−b | −/− | −/− |
| 8 | +/+ | +/+ | −/− | −/−b | −/− | −/− |
+/+ (dark green) indicates positive in both the bulk repertoire and the gDNA data for predicted single nucleotide polymorphisms (SNPs); −/− (light green) indicates negative in both the bulk repertoire and the gDNA data for predicted SNPs; −/N.D. (yellow) indicates negative in bulk repertoire data but gDNA failed to amplify during gDNA validation for predicted SNPs.
aIndicates the existence of copy-number variants with more than two alleles detected in the gDNA data that belong to the same gene.
bIndicates the gene was not detected in the repertoire or gDNA, possibly due to gene deletion.